Studies on the nutrition and physiology of Pasteurella pestis. I. A chemically defined culture medium for Pasteurella pestis.

The chemically defined culture media for Pasteurella pestis which were formulated by Rao (1939, 1940a, b), Berkman (1942), Doudoroff (1943), and Herbert (1949) did not support the growth of any of the 27 strains available in this laboratory. This report describes the.development of a chemically defined medium which was evaluated in terms of the following criteria: yields of viable cells, minimum size of inoculum, suitability for maintamin g virulence on repeated subculture, and as a solid medium.